Cryopreserved versus freshly isolated lymphocytes in human biomonitoring: endogenous and induced DNA damage, antioxidant status and repair capability
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چکیده
منابع مشابه
Studies on electron beam induced DNA damage and repair kinetics in lymphocytes by alkaline comet assay
Background: Exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and DNA through radiolysis of cellular water. Since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the DNA damage and repair kinetics would help to predict the radiation sensitivity of cel...
متن کاملRepair of ionizing radiation induced DNA damage in human lymphocytes.
Phytohemagglutinin stimulated human lymphocytes exhibit a 20 fold increase in DNA repair synthesis following ionizing radiation damage compared to the level of repair in unstimulated cells. The peak of repair synthesis coincides with that for DNA replication. Stimulated lymphocytes provide a relatively simple assay for ionizing radiation repair defects.
متن کاملEvaluating the effects of galbanic acid on hydrogen peroxide-induced oxidative DNA damage in human lymphocytes
Objective: Ferula szowitsiana has been widely used for medicinal purposes around the world. The anti-oxidant effect of F.szowitsiana had been proved. The current study aims to determine the protective effects of galbanic acid, a sesquiterpene coumarin from F. szowitsiana, against hydrogen peroxide (H2O2) - induced oxidative DNA damage in human lymphocytes. Materials and Methods: Human lymphocyt...
متن کاملstudies on electron beam induced dna damage and repair kinetics in lymphocytes by alkaline comet assay
background: exposure to ionizing radiation is known to induce oxidative stress followed by damage to critical biomolecules like lipids, proteins and dna through radiolysis of cellular water. since radiation has been widely used as an important tool in therapy of cancer, the detailed investigation regarding the dna damage and repair kinetics would help to predict the radiation sensitivity of cel...
متن کاملRepair of radiation induced DNA damage in human hematopoietic stem and progenitor cells and mature lymphocytes
Hematopoietic stem and progenitor cells (HSPC) are responsible for the constant renewal of all mature blood cells (lymphocytes, PBLs included). In view of the increasing application of heavy ions in radiotherapy [1, 2] and as HSPC may be located in the radiation field during radiotreatment, their radiobiological features and those of their descendents have to be investigated. Since the effect o...
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ژورنال
عنوان ژورنال: Mutagenesis
سال: 2002
ISSN: 1464-3804
DOI: 10.1093/mutage/17.3.211